In order to explore more efficient protein substitutes by improving the utilization of non-protein nitrogen compounds in ruminants, the experiment was undertaken. The e(facts of sulfur coated urea (SCU) and diuredio isobutane (DUIB) in the luminal fluid on the concentration of NH₃-N, the total count of the ruminal ciliates and pH value were estimated in vitro.
The results obtained from the experiment were as follows:
1. The pH of the media of the group added diuredio isobutane and sulfur costed urea tends to decrease slightly at 9 hours after the incubation, but no pH changes were observed in the media added urea alone .
2. The number of the ciliates in the luminal fluid was slightly increased 9 hours after the incubation in all groups.
3. The concentrations of NH₃-N in the ruminal fluid were gradually increased according to the incubation in vitro showing 418, 431 and 627 ㎍/㎖ in the group added diuredio isobutane and 428, 569 and 792㎍/㎖ in the group added sulfur coated urea at 0, 0.5 and 9 hours after the incubation, respectively.
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